A total of sixty five neonates suspected to be septicaemia were sampled. Nine Gram negative organisms were isolated out of the 40 samples with positive blood culture accounting for 22.5% of the bacterial organisms responsible for neonatal septicaemia within the period of study. Of this number, the most prevalent was Enterobacter cloacae, Enterobacter cloacae accounting for 4 out of a total of 40 patients (10%) and 44.44% of the total Gram negative organisms.  Klebsiella pneumoniae was next with an incidence of 7.5% which represents 33.33% of the gram negative organisms. Citrobacter freundii and Escherichia coli had incidence of 2.5% each constituting 11.11% of the total gram negative organism respectively. Incidence was higher in males 55.56% while in females it was 44.44%. The overall sensitivity percentage to ampicillin and gentamicin was 77.78 %, followed by nitrofurantoin , streptomycin and tetracycline which was 66.67%. the percentage sensitivity to colistin was 55.56% while sensitivity to cotrimazole and nalixidic acid was 44.44% respectively. All the Enterobacter isolates were sensitive to ampicillin but one isolate showed multiple resistance to the other antibiotic. the E coli strain also showed evidence of multiple resistance, being resistant to the ampicillin and gentamicin and streptomycin. It was however sensitive to tetracycline and nitrofurantoin.