ISSN: 0973-7510

E-ISSN: 2581-690X

Ahmed Abdel-Megeed1,4 , Ashour Eman3,4 and Al-Meshal Areej Suliman2
1Department of Plant Protection, Faculty of Agriculture, (Saba Basha), Alexandria University, Egypt.
2Department of Biology, College of Science &Humanity Studies, Salman bin Abdulaziz University, P.O.Box 292 AlKharj 11942, Saudi Arabia
3Department of Microbiology, Faculty of Agriculture, Mansoura University, Egypt.
4Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):501-508
© The Author(s). 2013
Received: 30/10/2013 | Accepted: 08/11/2013 | Published: 30/11/2013
Abstract

In the present investigation an attempt has been made to study glyphosate, Diclofop-methyl and Pendimethalin and fenvalerate induced changs in protein submits the herbicide-tolerant fungi isolated from pesticides polluted-soil. Severely loss in the total soluble cell ions (SCI) and total cell protein percentage (TCPC) concentrations was observed. For glyphosate, Aspergillus flavus (86.30%) was the most affected one, followed by Penicillium spiculisporus (76.30%), Penicillium verruculosum (64.40%) and Alternaria tenuissima (64%), respectively. The other fungal strains exhibited <50% in total SCI. For Pendimethalin, Alternaria tenuissima (54.01%) was the most affected fungi followed by Penicillium spinulosum (50.68%), respectively. The other fungal strains exhibited <50% in total SCI. For Diclofop-methyl, Penicillium spiculisporus (74.20%) was the most affected fungi followed by Aspergillus tamarii (70.67%), Aspergillus niger (56.16%), Aspergillus terreus (54.67%) and Alternaria tenuissima (58.82%), respectively. The other fungal strains exhibited d” 50% SCI. The loss of TCPC by Glyphosate, Alternaria tenuissima (64.71%) was the most effected fungi, followed by Penicillium spiculisporus (57.14%), respectively. The other fungal strains exhibited <50%. For Pendimethalin, A. terreus (54.29%) was the most affected fungi followed by Alternaria tenuissima (50.00%), respectively. The other fungal strains exhibited <50%. For Diclofop-methyl, Penicillium spiculisporus (60%), followed by Alternaria tenuissima (58.82%), Aspergillus tamarii (55.56%), respectively. The other fungal strains exhibited <50%. The results proved changes in protein profile induced in herbicide-tolerant fungi.

Keywords

Herbicides, Tolerance, Fungi, Polluted-soil, DNA, protein

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