ISSN: 0973-7510

E-ISSN: 2581-690X

Ankita Jain1, Mayank Rawat3, Soumendu Chakaravarti2, Abhishek1, Vinod Chaturvedi2, Losa Chesti1, Ankush K. Niranjan1, Rajat Varshney1 and Bablu Kumar2
1Division of Bacteriololy and Mycology, Indian Veterinary Research Institute, Bareilly – 243 122, India.
2Division of Biological Products, Indian Veterinary Research Institute, Bareilly – 243 122, India.
3Division of Biological Standardization, Indian Veterinary Research Institute, Bareilly – 243122, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2063-2070
© The Author(s). 2016
Received: 30/01/2016 | Accepted: 20/02/2016 | Published: 30/09/2016
Abstract

TLRs sense pathogen associated molecular patterns of invading microbes which initiate intracellular signaling cascades and lead to production of pro-inflammatory cytokines which in turn help in bridging innate and adaptive immune response. TLRs and cytokines response of plain and alum adsorbed lysate of Brucella abortus strain 19 in mice model were evaluated by relative expression of the transcript by real time PCR. Three different groups of mice immunized with plain lysate bacterin, alum adjuvanated lysate, standard B. abortus strain 19 vaccine respectively and an unimmunized control group were used in present study. The results showed higher expression of TLR2, TLR4, TLR9, IL-4, IL-12 and INF-a transcripts in spleen of all the immunized mice groups as compared to unimmunized control groups. The relative higher transcripts expression of TLRs and cytokines in alum adjuvanated lysate as comparison to plain lysate bacterin suggest better immuno-protective response of alum adjuvanated phage lysate bacterin against the brucellosis.

Keywords

Brucella phage lysate, murine, TLRs, Cytokines.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.