Studies on Biotransformation of rifamycin B to rifamycin S revealed that immobilized mycelia of Curvularia lunata are more efficient than free cells of organism with respect to time, cultural conditions and cost. Effect of various polymers revealed that carboxymethylcellulose has stimulatory effect on biotransformation whereas other polymers do not show significant effect on biotransformation. TLC analysis of transformed product shows two bands, one matching with standard rifamycin B while another with standard rifamycin S indicates significant transformation. Optimum transformation takes place at 400c and pH7 respectively. UV-Visible spectrophotometric studies indicated that rifamycin B and rifamycin S has absorption maxima 425 and 525 respectively.
Biotransformation, rifamycin B, rifamycin S, TLC, Curvularia lunata
Share This Article
© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.