ISSN: 0973-7510

E-ISSN: 2581-690X

Pinkham Vongphachanh1, Weerasak Saksirirat1,2,3 and Suwita Seapaisan1,2,3
1Plant Pathology Section, Department of Plant Science and Agricultural Resources,
Faculty of Agriculture, Khon Kaen University, Khon Kean 40002, Thailand.
2Agricultural Biotechnology Research Center for Sustainable Economy,
Khon Kaen University, Khon Kaen 40002, Thailand.
3Center of Agricultural Biotechnology, Khon Kaen University, Science and Technology Postgraduate Education and Research Development Office (AG-BIO/PERDO-CHE), Thailand.
J. Pure Appl. Microbiol., 2016, 10 (3): 1735-1744
© The Author(s). 2016
Received: 17/06/2016 | Accepted: 02/08/2016 | Published: 30/09/2016
Abstract

The objective of this study, aims to select the effective antagonistic fungi to control sheath diseases of rice. The experiments were conducted in the laboratory and green house. In the laboratory, the antagonist fungi, Trichoderma hazianum T9, T18 and T13 T. asperellum and T. koningii isolate 67 were tested on their efficiency against R. solani and R. oryzae in dual cultures. The result showed that the isolates T9, T13, T18, 67 were able to inhibit the colony of R. oryzae and R. solani. The percent of colony inhibition was highly efficient against R. oryzae isolates VHN, VHN1-3 and SMN1-7 as 88%, 63% and 63%, respectively. For R. solani, isolates VNN, SMC1-2 and SMN1-5 exhibited the percent of inhibition as 41%, 40% and 36%, respectively. In the green house test, the result revealed the 3 effective isolates, T13, T18, 67 for control R. oryzae. The best method for application of isolates T18 and T13 were foliar spray, by which disease suppression was 92% and 87%, respectively. Isolate 67 was applied by root dip method and causing disease suppression of 87%. For R. solani, the isolate T18 was applied by soil treatment and expressed disease suppression of 61%. The best method for application of isolates T13 and 67 were the combination of soil treatment, root dip and foliar spray, by which disease suppression was 60% and 50%, respectively. All 3 isolates were significant difference (P<0.05) in disease suppression compared to control. This study suggests the biological control approach for sheath diseases of rice in Lao PDR.

Keywords

Sheath diseases, Rhizoctonia solani, Rhizoctonia oryzae,
Trichoderma spp, biological control.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.