ISSN: 0973-7510

E-ISSN: 2581-690X

Gurupad B. Balol and M.S. Patil
1Department of Plant Pathology , University of Agricultural Sciences, UAS., Dharwad, Karnataka, India.
J Pure Appl Microbiol. 2014;8(1):749-752
© The Author(s). 2014
Received: 08/05/2012 | Accepted: 14/06/2012 | Published: 28/02/2014

Tomato bud blight disease caused by Groundnut bud necrosis virus (GBNV) belongs to genus Tospovirus of the Bunyaviridae. This is economically important disease  affecting tomato crop. Virus causes, necrotic spots on leaves petiole and stems, chlorotic spots on fruits, bud blight and  finally wilting of the plants. Virus was mechanically transmitted on to  cow pea cv. C152. leaves and produced chlorotic as well necrotic local lesions. Reverse transcription-polymerase chain reaction (RT-PCR) was used for studying the presence of GBNV  in different parts, i.e., roots,  midrib, fruit  pericarp and leaves of tomato bud blight field sample. Amplification of 831bp GBNV-CP by RT-PCR with degenerate primers  revealed the positive reaction for GBNV infection in different parts, i.e., roots,  midrib, fruit  pericarp and leaves of tomato.


GBNV, GBNV-CP, RT-PCR, Detection, Bud blight

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