Tomato bud blight disease caused by Groundnut bud necrosis virus (GBNV) belongs to genus Tospovirus of the Bunyaviridae. This is economically important disease affecting tomato crop. Virus causes, necrotic spots on leaves petiole and stems, chlorotic spots on fruits, bud blight and finally wilting of the plants. Virus was mechanically transmitted on to cow pea cv. C152. leaves and produced chlorotic as well necrotic local lesions. Reverse transcription-polymerase chain reaction (RT-PCR) was used for studying the presence of GBNV in different parts, i.e., roots, midrib, fruit pericarp and leaves of tomato bud blight field sample. Amplification of 831bp GBNV-CP by RT-PCR with degenerate primers revealed the positive reaction for GBNV infection in different parts, i.e., roots, midrib, fruit pericarp and leaves of tomato.
GBNV, GBNV-CP, RT-PCR, Detection, Bud blight
Share This Article
© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.