Nowadays medicinal plants characterized by great stature where it is one of the main resources of medicinal drugs. The study chooses the Anisotes trisulcus as a platoon of alakancih as medicinal plant to conduct the studies of this research on chemical analysis and the effect of plant extracts as antibacterial and antifungal after extraction by cascade separation by several solvents, then study the effects of this plant on sugar level in the blood by giving the plant as a medicine to experimental mice have been struck with diabetes, and detect the effect of plant extract on group of other blood characteristic. The extraction process conducted on the plant and the process of separation and distinguishing the plant component by GC.MS instrument. By studying the antioxidant effect of plant extract, the stem extract has the biggest inhibition effect on free radicals, followed by leaves then roots extract. As for the effect of aqueous extract of the plant on level of sugar in blood, the experiments which were held on diabetic experimental mice show that the effect was clear by lowering sugar level from 267.3 to 141.39 mg/dl, which means important indication for validity of this extract as treatment of high levels of sugar in blood. At the same time, the effect of this extract led to controlling many of diabetic experimental mice blood characteristics and returned it to near numbers of the normal level like white blood cells, Hemoglobin concentration, number of platelets, and percentage of granulocytes.