ISSN: 0973-7510

E-ISSN: 2581-690X

Pornpun Siramon1 and Klanarong Sriroth2
1King Mongkut’s University of Technology Thonburi (Ratchaburi Campus), Ratchaburi, 70150, Thailand.
2Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
J. Pure Appl. Microbiol., 2016, 10 (3): 1781-1786
© The Author(s). 2016
Received: 14/06/2016 | Accepted: 03/08/2016 | Published: 30/09/2016

The objective of this study is to examine the potential applications of glycerol, a by-product derived from jatropha biodiesel production, via bioconversion into ethanol by Enterobacter aerogenes TISTR 1468.  The purity of crude glycerol sample can be enhanced using sulfuric acid in varying concentrations.  It was found that the addition by weight of 5% sulfuric acid could increase the glycerol purity from 27.68% to 94.43% with 65.42% of glycerol yield. Once purified, the glycerol sample was applied at 30 g/L to form the carbon substrate required in the anaerobic fermentation process by which ethanol can be obtained through the use of E. aerogenes for 120 hours at a constant 37 °C. Under these conditions, purified glycerol yielded 8.39 g/L ethanol while crude glycerol yielded 6.54 g/L ethanol. The ethanol produced via this approach was shown to be highly efficient in comparison with commercial glycerol with purity at 99.5%, which produced 7.46 g/L ethanol under the same condition.


Biodiesel production, Crude glycerol, Enterobacter aerogenes, Ethanol, Jatropha curcas L

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.