Bioconversion of Cassava Starch to Ethanol in a Simultaneous Saccharification and Fermentation Process by Co-Cultures of Aspergillus niger and Saccharomyces cerevisiae

ISSN: 0973-7510

E-ISSN: 2581-690X

Bioconversion of Cassava Starch to Ethanol in a Simultaneous Saccharification and Fermentation Process by Co-Cultures of Aspergillus niger and Saccharomyces cerevisiae

S.A. Ado¹ , G.B. Olukotun¹, J.B. Ameh¹ and A. Yabaya²

¹Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.

²Department of Microbiology, Kaduna State University, Kaduna, Nigeria.

J Pure Appl Microbiol. 2010;4(1):55-61

Received: 02/07/2009 | Accepted: 20/10/2009 | Published: 30/04/2010

Abstract

Ethanol production by co-cultures of A. niger (GS4) and S. cerevisiae (BK6) was studied using cassava starch as substrate. At 1% substrate concentration ethanol yield was 0.35g/100ml while the ethanol concentration increased to a maximum of 3.60g/100ml at 8% substrate concentration. When the culture conditions were optimized, the ethanol yield further increased to 4.30g/100ml at a temperature of 35^oC, pH 5.0, 300rpm agitation rate and reduced fermentation period of 4 days.

Keywords

A. niger, S. cerevisiae, Fermentation, Ethanol, Cell dry weight, Residual sugar

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