ISSN: 0973-7510

E-ISSN: 2581-690X

Poornima1 , Gururaj Sunkad1, Hari K. Sudini2 and M.K. Naik1
1Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584 104, Karnataka, India.
2International Crops Research Institute for the Semi-Arid Tropics Patencheru, Hyderabad, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1511-1517
© The Author(s). 2016
Received: 12/02/2016 | Accepted: 12/03/2016 | Published: 30/06/2016
Abstract

Peroxidases (POD), poly phenol oxidases (PPO) and phenyl alanine ammonia lyase (PAL) from healthy and Sclerotium rolfsii inoculated stems of 6 genotypes of groundnut were estimated after 3 days of inoculation. The activity of PO, PPO and PAL began from 3 rd DAI and gradually increased up to 6 th DAI and thereafter declined.  PO, PPO, and PAL activity was more in infected stem sample compared to healthy stem. Among the genotypes resistant and moderately resistant genotypes recorded maximum PO, PPO and PAL activity whereas susceptible genotypes recorded less activity. Further, biochemical constituents of healthy and stem rot infected tissues of different genotypes of groundnut were evaluated. The results revealed that total sugar content was more in resistant genotypes (12009 and 17110), moderately resistant genotypes (17159 and 17169) when compared to highly susceptible genotypes (TMV-2 and KRG-1). Further, it is also found that healthy stems of groundnut recorded more sugar, reducing and non reducing sugar content compared to diseased stems. Phenol and protein contents in healthy and infected stems varied among genotypes. Phenol and protein contents were more in infected stem as compared to healthy stem. Further, resistant and moderately resistant genotypes showed more contents of phenol and protein than susceptible genotypes.

Keywords

Groundnut, Sclerotium rolfsii, Biochemical constituents, Defense enzymes.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.