ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ali Jalil Obaid1, Jawad K. Abood Al-Janabi2 and Wijdan Ridha Taj-Aldin3
1Department of Biology, College of Science, University of Babylon, Hilla University Collage, Babylon –Hilla, Iraq.
2Department of Biology, College of Science, University of Babylon, Al-Mustaqbal University College, Babylon – Hilla, Iraq.
3Department of Biology, College of Science, University of Babylon, Babylon – Hilla, Iraq.
J. Pure Appl. Microbiol., 2020, 14 (1): 615-626 | Article Number: 5721
https://doi.org/10.22207/JPAM.14.1.64 | © The Author(s). 2020
Received: 17/07/2019 | Accepted: 18/02/2020 | Published: 11/03/2020
Abstract

Trichophyton rubrum is the most common cause of dermatophyte skin infections in humans worldwide. The aim of the current study is to search for effective and safe antifungal agents by investigating the bioactivity of Pimpinella anisum and Moringa oleifera extracts, including the impact on the keratinase gene expression of T. rubrum. Astragalin and cryptochlorogenic acid were detected in M. oleifera leaf extracts by Gas chromatography-mass spectrometry (GC-MS), whereas anethole was extracted from P. anisum oil. The expression of the keratinase gene was assessed in two strains of T. rubrum (KP979791 and KP979787) in Sabouraud Dextrose Broth (SDB) medium (containing 10% keratin) separately supplemented with plant extracts fractions. The results showed that cryptochlorogenic acid and astragalin were active compounds (molecular weights of 353.26 and 449.35 g/mol, respectively that considerably down-regulated the keratinase genes in both strains of T. rubrum. M. oleifera crude extract, astragalin, cryptochlorogenic acid, anise oil, and anethole resulted in the down-regulation of the keratinase genes of T. rubrum strain KP979791 by 0.048, 0.291, 0.001, 0.007, and 0.009 (fold-change), respectively, and that of strain KP979787 by 0.151, 0.42, 0.16, 0.079, and 0.092 as compared to the control (1.00). Nearly all of these outcomes are novel findings that may aid in the identification of novel effective drug targets and antifungal agents.

Keywords

Trichophyton rubrum, Moringa oleifera, Pimpinella anisum, Chromatography, keratinase gene.

Article Metrics

Article View: 288
PDF Download: 70

Share This Article

© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.