ISSN: 0973-7510

E-ISSN: 2581-690X

K. Karthiga Devi, K. Moorthy, N. Thajuddin, S. Boobathy and P. Prabakaran
Vivekanandha College of Arts & Sciences for Women, Elayampalayam, Tiruchengode – 637 205, India. Easma Institute of Technology, Aravakurichy, India.
J Pure Appl Microbiol. 2011;5(2):997-1001
© The Author(s). 2011
Received: 20/02/2011 | Accepted: 14/04/2011| Published: 31/10/2011
Abstract

Bacteriocin producing Lactobacillus brevis strain isolated from raw cow milk showed high range of antimicrobial activity against some food borne pathogens such as Brevibacillus brevis, Proteus vulgaricus, Salmonella typhi and Pseudomonas sp. Maximum growth of Lactobacillus brevis strain was found in 36th hour culture.  The bacteriocin has purified by column chromatography (DEAE cellulose). The purified Bacteriocin like substances was identified as 29 K Da peptide by SDS PAGE. The study revealed the possibility of using bacteriocin as a food preservative and the Lactobacillus brevis strain as a probiotic. Cellulose production was estimated by Upedegraff method.

Keywords

Bacteriocin, Cellulose production, Lactic acid bacteri, Raw cow milk

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