Bacteriocin producing Lactobacillus brevis strain isolated from raw cow milk showed high range of antimicrobial activity against some food borne pathogens such as Brevibacillus brevis, Proteus vulgaricus, Salmonella typhi and Pseudomonas sp. Maximum growth of Lactobacillus brevis strain was found in 36^th hour culture.  The bacteriocin has purified by column chromatography (DEAE cellulose). The purified Bacteriocin like substances was identified as 29 K Da peptide by SDS PAGE. The study revealed the possibility of using bacteriocin as a food preservative and the Lactobacillus brevis strain as a probiotic. Cellulose production was estimated by Upedegraff method.