ISSN: 0973-7510
E-ISSN: 2581-690X
The purpose of the present study is to evaluate three method for detection of biofilm formation in Enterococcus spp. for detection of biofilm formation, 150 clinical isolates of Enterococcus spp. were observed by Tissue culture plate (TCP), Tube method (TM) and Congo Red agar ( CRA) method. Out of the 150 Enterococcus spp. 98 ( 65%) displayed a biofilm positive phenotype under the optimized condition. TCP method were further classified as high 28(18.66%), moderate 50(33.33%) and weak 72(48%) isolates or non biofilm producer. During observation it was seen that TM and CRA did not correlated with TCP method for detection of biofilm formation in Enterococcus spp. The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm production by Enterococci.
Enterococci, Biofilm, Pathogenecity, Virulence, Transcriptional regulator, Intrinsic resistance
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