ISSN: 0973-7510

E-ISSN: 2581-690X

Priyanka Bordoloi, Krishna Sharma, Rajib Sharma and Sutopa Das
1College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati – 781 022, India.
J Pure Appl Microbiol. 2014;8(4):3367-3372
© The Author(s). 2014
Received: 12/11/2013 | Accepted: 28/02/2014 | Published: 31/08/2014
Abstract

Foot-and-mouth disease (FMD) is a highly contagious and economically important disease of cloven footed domesticated and wild animals. Lot of works have been carried out to develop diagnostic tests in regard to this disease The present study was undertaken for detection of FMDV serotype ‘O’ in infected as well as in recovered animals by using sandwich ELISA, virus isolation in cell culture in BHK-21 cell line and reverse transcription PCR and the finding were compiled together to see the most sensitive test for detection of FMDV type ‘O’. A total of 50 clinical samples comprising 40 tongue epithelium, 6 oral swab and 4 throat swab samples were subjected to sandwich ELISA where 20 were found positive for FMDV type ‘O’ (40%),  where as all the swab samples were found to be negative for any serotype of FMDV. Sandwich ELISA positive samples and all the swab samples were subjected to virus isolation where only 11 samples showed CPE (36.66%). FMD virus serotype ‘O’ could be detected in all samples (100%), by RT-PCR using serotype ‘O’ specific primer. Therefore, the present study revealed that RT-PCR is most sensitive for the detection of RNA of FMD virus when compared to the results of the three tests.

Keywords

FMD, sandwich ELISA, CPE, RT-PCR

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.