ISSN: 0973-7510
E-ISSN: 2581-690X
Main medium components that influence whole-cell lipase from A. Niger GZUF36 catalyzing synthesis of 1,3-diglyceride (1,3-DG) by glycerolysis of triglyceride (TG) are identified by Plackett-Burman (PB) design. The response index was yield of DG synthesized by the lipase-catalyzed glycerolysis. The selected factors for PB design included glucose, soybean meal, zinc sulfate, disodium hydrogen phosphate, calcium chloride, potassium chloride and potassium hydrogen phosphate. The results showed that the main medium constituents were glucose, soybean meal and potassium chloride. Other factors were not significant. On the base of PB design, the optimized medium was obtained, with which the yield of diglyceride (DG) was increased 0.78-fold compared with basal medium. The synthesized DG can be used as healthy lipid. This study would improve the further optimization steps on the bioprocess development tracks.
Plackett-Burman design, diglyceride, A. niger GZUF36, Main medium constituents
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