ISSN: 0973-7510

E-ISSN: 2581-690X

Punyisa Charirak1, Weerasak Saksirirat1,2 , Sanun Jogloy3 and Suwita Saepaisan1,2
1Section of Plant Pathology, Department of Plant Science and Agricultural Resources,
Faculty of Agriculture, Khon Kaen University, Khon Kaen – 40002, Thailand.
2Agricultural Biotechnology Research Center for Sustainable Economy, Khon Kaen University, Khon Kaen – 40002, Thailand.
3Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University, Khon Kaen – 40002, Thailand.
J. Pure Appl. Microbiol., 2016, 10 (2): 853-863
© The Author(s). 2016
Received: 13/02/2016 | Accepted: 09/03/2016 | Published: 30/06/2016

Jerusalem artichoke (Helianthus tuberosus L.) is a multipurpose crop grown for bio-fuel, feed additive and functional food products. Stem rot disease caused by Sclerotium rolfsii Sacc. is an important threat for Jerusalem artichoke production in the tropics, and disease control using induced resistance strategy is worth exploring. The objective of this study were to investigate the induction of chitinase and b-1,3-glucanase activities in leaves of H. tuberosus after treated with microorganisms, Bacillus firmus BSR 032, Trichoderma harzianum T9 and mycorrhizal fungus, Glomus clarum and to evaluate their efficacy on controlling the disease under green house condition. Increase in chitinase and b-1,3-glucanase activity and reduction in disease incidence were observed for the plants treated with these antagonistic microorganisms. The highest and most consistent increase in chitinase and b-1,3-glucanase was observed in the plants treated with T. harzianum T9 followed by T. harzianum T9+G. clarum, and b-1,3-glucanase was found at higher activity than chitinase. The T. harzianum T9 could reduced the disease incidence of 44.4%, followed by T. harzianum T9+G. clarum (22.8%), B. firmus BSR 032+G. clarum (15%), B. firmus BSR032 (8.6%) and G. clarum (4.2%), respectively. This T. harzianum T9 is a promising antagonistic microorganism for controlling stem rot disease.


Antagonistic microorganisms, Chitinase, Disease control, Disease incidence, 1,3-glucanase.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.