ISSN: 0973-7510

E-ISSN: 2581-690X

Sevil Toroglu1, Hatice Avan1 and Dilek Keskin2
1Department of Biology, Faculty of Arts and Science, Kahramanmaras Sütcü Imam University, 46100- Kahramanmaras, Turkey,
2Cine Vocational High School, Adnan Menderes University, 09500- Cine-Aydin, Turkey.
J Pure Appl Microbiol. 2014;8(2):985-991
© The Author(s). 2014
Received: 10/06/2013 | Accepted: 14/08/2013 | Published: 31/04/2014
Abstract

To determine the level of resistance to the widely used antibiotics in clinical isolates of Esherichia coli. 74 isolates were collected from different hospitals in Kahramanmaras and recorded at specimens. Antibiotic resistance was determined by agar disc diffusion method using Mueller-Hinton agar according to Clinical and Laboratory Standards Institute recommendations and the production of β-lactamase was detected with the iodometric slide test. This study was carried out in the laboratory of Kahramanmaras Sütcü Imam University, Biology Department. The results indicated that resistance rate of antibiotics was in the range of 91% Penicilin(PEN), 65% Tetracycline (TET) and Amoxicillin (AMX), 62% Cefazolin(CEFX), 59% Streptomycine( STR), 34% Oflaxain (OFL), 32% Chlorampenicol (CHL) and Ceftriaxone (CEFT), 27% Gentamycin (GEN), 24% Cefoxitin(CEFX), 18% Nitrofurantoin(NIT), %12 Meropenem(MER). Among the 74 isolates of E.coli were showed 38(51%) beta lactamase activity and 36(49%) isolates of E.coli were showed no beta lactamase activity. Out of 74 isolates, 50(68%) isolates showed Multiple Antibiotic Resistance three to twelve antibiotics.

Keywords

Esherichia coli, Antibiotic, Resistance, Beta lactamase activity

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