ISSN: 0973-7510

E-ISSN: 2581-690X

Sumaira Sharif1, Muhammad Shahid1 , Munir Ahmad Sheikh1 and Sajjad ur Rehman2

1Bioassays Section, Medicinal Biochemistry Lab., Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan.
2Institute of microbiology, University of Agriculture Faisalabad, Pakistan.
J. Pure Appl. Microbiol. 2014, 8(6):4565-4571
© The Author(s). 2014
Received: 07/10/2014 | Accepted: 28/11/2014 | Published: 31/12/2014

Antimicrobial potential of different mushrooms extracts sequentially isolated by n– hexane, dichloromethane, ethyl acetate and water from four cultivated, Pleurotus ostreatus, Lentinus edodes, Volvoriella volvacea, Hericeium erinaceus and one wild Ganoderma lucidum were evaluated for their ability to inhibit the growth of different microbial species. Antimicrobial activity was assayed using disc diffusion and micro dilution methods. Antimicrobial potential was screened against two Gram negative bacterial species (Escherichia coli, Pasturelaa multocida) two Gram positive (Bacillus subtilis, Staphylococcus aureus) and four fungal species (Aspergillus niger, Aspergillus flavus, Fusarium solani, Helmenthosporium mydis), all the fractions possessed considerable antimicrobial activity, the fractions of V. volvacea and H. erinaceus were more effective against tested microbial species and can be compared favorably with positive controls (Rifampicin and fluconazole). Ethyl acetate and water fractions of V. volvacea and H. erinaceus showed zone of inhibition 15 ± 0.4 mm and 16 ± 0.6 mm against P. Multocida and B. subtilis respectively with minimum inhibitory concentration values of 294 and 255 ug/mL respectively. The maximum antifungal was again observed in H. erinaceus and V. volvacea. The fractions were more effective against A. flavus as compare to other fungal species with maximum zone of inhibition ranging from 9 ± 0.5-14 ± 0.9 mm and MIC values 302 ± 0.53-660 ± 0.73 ug/mL. In conclusion, the analyzed mushrooms extract are promising source of antimicrobial agents.


Mushrooms, Antimicrobial activity. Pleurotus ostreatus, Volvoriella volvacea Lentinus edodes

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