ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Abdulhakim Bawadekji1 , Mohd. Imran2 and Mohammad Akram Randahawa3
1Department of Biological Sciences, College of Science, Northern Border University, P.O. Box 1321, Arar 91431, Saudi Arabia.
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Northern Border University, P.O. Box 840, Rafha 91911, Saudi Arabia.
3Department of Pharmacology, Hazrat Bari Sarkar Medical & Dental College, Tarlai 45550, Islamabad, Pakistan.
J. Pure Appl. Microbiol., 2019, 13 (4): 2189-2194 | Article Number: 5888 | © The Author(s). 2019
Received: 28/09/2019 | Accepted: 29/10/2019 | Published: 11/12/2019

The purpose of this work was to perform the phytochemical and antimicrobial activity evaluation of the water-immiscible solvent extracts of Olive tree leaves (Olea europaea L., Family: Oleaceae). Seven sample extracts of the leaves of O. europaea were obtained using dichloromethane (DCM), dichloroethane (DCE) along with their mixtures with chloroform (CH), and ethyl acetate (EA). The phytochemical studies were carried out using the standard procedures. Serial plate dilution technique was used to perform the antimicrobial activity of the extracts. The phytochemical tests revealed the presence of steroids, terpenoids, saponins and flavonoids in all extracts of olive leaves. The 1:1 mixture of ethyl acetate and dichloromethane (EA:DCM) exhibited equivalent MIC (20 µgml-1, 100%) values concerning Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa with respect to ofloxacin (MIC = 20 µgml-1, 100%). Similarly, the 1:1 mixture of ethyl acetate and dichloroethane (EA:DCE) exhibited equivalent MIC (20 µgml-1, 100%) values concerning E. coli and P. aeruginosa with respect to ofloxacin (MIC = 20 µgml-1, 100%). The 1:1 mixture of EA:DCM and EA:DCE showed moderate antifungal activity (MIC = 20 µgml-1, 75%) with respect to fluconazole against Aspergillus niger and Candida albicans. The water immiscible solvent extracts showed more potent antimicrobial activity than the water extract of the olive leaves, which might be because of the presence of the lipophilic compounds in the water immiscible solvent extracts of the olive leaves. It is also expected that by increasing the concentration of ethyl acetate in EA:DCM & EA:DCE solvent system may further provide better MIC values.


Olive leaves, Water immiscible solvent, Extract, Antimicrobial.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.