ISSN: 0973-7510

E-ISSN: 2581-690X

M. Bhavana1, VSSL Prasad Talluri1, K. Sivakumar1, P. Anil Kumar2 and S.V. Rajagopal1

1Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam 530045, India.
2Microbial Type Culture Collection and Genebank, Institute of Microbial Technology, Chandigarh 160036, India.
J. Pure Appl. Microbiol. 2014, 8(6):4681-4686
© The Author(s). 2014
Received: 20/07/2014 | Accepted: 10/09/2014 | Published: 31/12/2014
Abstract

A new potent antagonistic actinomycetes strain was isolated from marine sediment samples of Visakhapatnam sea coast at Bay of Bengal, India. The isolate grows on starch casein agar medium with creamy-white substrate mycelium and blackish-gray aerial mycelium. On the basis of cultural characteristics, biochemical, chemotaxonomic tests and phylogenetic studies based on 16S rRNA gene sequence it was identified as Streptomyces carpaticus sp and deposited in microbial type culture collection IMTECH Chandigarh India with an accession number of  MTTC-11062.  The isolate showed broad spectrum antimicrobial activity against the pathogenic bacterial and fungal species studied and also inhibited the proliferation of HeLa and HepG2 cell lines with an IC50 values of 15.04 ¼g/ml and 18.36¼g/ml respectively. The survival rate of HepG2 cell lines was higher than of HeLa cell lines at the same concentration of antibiotic.

Keywords

Marine sediment, Actinomycetes, Streptomyces carpaticus, Antimicrobial activity, antiproliferative activity, 16SrRNA

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