ISSN: 0973-7510

E-ISSN: 2581-690X

Vishal Khandelwal , Ashok Kumar Bhatia and Anjana Goel
Department of Biotechnology, GLA University, Mathura – 281 406, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 209-216
© The Author(s). 2016
Received: 10/01/2016 | Accepted: 06/02/2016 | Published: 31/03/2016
Abstract

Hot aqueous extract (HAE) of leaves of Anthocephalus cadamba (Roxb.) Miq, Rubiaceae has been screened for antimicrobial and antioxidant activity. Leaf extract was tested against some gram positive, gram negative and certain fungal strains. Leaf extract showed significant (p < .01) antibacterial and antifungal activity against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aureginosa and Aspergillus niger. Ofloxacin and amphotericin-B were used as standard with respect to bacteria and fungi. MIC of leaf extract was 0.039 mg/ml for S. aureus, 0.078 mg/ml for B. cereus whereas, 0.156mg/ml for E. coli, P. aureginosa and A. niger. In-vitro antioxidant activity of the extract was evaluated by 2,2-diphenylhydrazyl free radical scavenging assay. Significant (p < .01) antioxidant activity in term of percentage inhibition of free radicals by the extract was found at 0.25 mg/10ml, 0.50 mg/10ml and 1.0 mg/10ml concentrations when compared with standards (ascorbic acid and butylated hydroxyl toluene).

Keywords

Anthocephalus cadamba, Antimicrobial activity, Phytochemicals, Antioxidant, DPPH free radical scavenging assay.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.