ISSN: 0973-7510

E-ISSN: 2581-690X

N.A.H. Yusoff1, M.S. Bustamam1, F. Abas1,2, A. Khatib3 and Y. Rukayadi1,2
1Laboratory of Natural Product, Institute of Bioscience, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
2Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
3Department of Pharmaceutical Chemistry,Kulliyyah of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang Darul Makmur, Malaysia.
J Pure Appl Microbiol. 2014;8(5):3681-3688
© The Author(s). 2014
Received: 07/05/2014 | Accepted: 21/07/2014 | Published: 31/10/2014
Abstract

The antimicrobial activity of Cosmos caudatus extract was evaluated against Bacillus cereus(ATCC 33019), Bacillus subtilis(ATCC 6633), Proteus mirabilis (ATCC 21100), Pseudomonas aeruginosa (ATCC 9027) and Candida albicans (ATCC 10231) using the methods as recommended by the Clinical and Laboratory Standard Institute (CLSI). The antimicrobial tests were conducted in term of susceptibility, minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration (MBC/MFC) and killing-time curve. The results showed that C. caudatus extract was susceptible against all tested pathogens; the inhibition zone ranged from 8.60 mm to 9.83 mm. The MIC and MBC/MFC values were ranged from 6.25 mg/ml-12.50 mg/ml and 12.50 mg/ml-50.00 mg/ml, respectively. Mean while, killing-time curves showed that C. caudatus extract can killed the B. cereus, B. subtilis, P. mirabilis, P. aeruginosa and C. albicans at concentration of 8 MIC for 2 h, 4 MIC for 2 h or 2 MIC for 2 h, 8 MIC for 4 h, 4 MIC for 0.5 h and 4 MIC for 1 h, as respectively. Findings indicated that C. caudatus extract has the potentiality to develop as a natural antimicrobial agent.

Keywords

Antimicrobial activity, Cosmos caudatus, Foodborne pathogen, Time-kill study, Plant extract

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.