One of the important human pathogens that can cause systemic infections is Candida albicans. Iron oxide Nano-particles with unique magnetic properties have a high potential for use in several biomedical, bioengineering and in vivo applications. In this study, we were investigated antifungal property of Iron Oxide Nano-particles. Candida albicans was undertaken to evaluate the dose- and time-dependent toxic potential. MTT assay showed 65% viability of Candida albicans in lower concentrations (5-30 µg/mL) and up to 12 hours of exposure, whereas at higher concentrations (30-100 µg/mL) and prolonged (24-48 hours) exposure viability reduced to 80%–95%. Our results  suggest that antifungal activity of colloidal Fe₃O₄ NP that can be useful for medical equipment  such  as  catheters,  implants,  denturescan  be  inhibited especially growth of Candida albicans.