Infections of the urinary tract are involved with the common infectious diseases in the population, by the yearly occurrence of 18 per 1000 people. This study carried out to determine the causative pathogenic bacteria of the urinary tract infections (UTIs) and to measure the frequency and susceptibility outline of different bacteria causing UTIs that acquired by the community in Basra city south Iraq. This study recruit102 persons (77 females with25 males) with the age range of (15-55) years old, clinically suspected for UTI. Clean midstream urine gathered from the patients in a sterile tube and tested by the use of typical procedures. Kirby-Bauer technique used to test antibiotic sensitivity of the isolated pathogenic bacteria according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Examination of centrifuged urine samples microscopically revealed 42 (31 females with 11 males) had pyuria (a significant number of pus cells) while 50 (37 females with 13 males) had scarcer pus cells. Eight urine samples (6 females and 2 males) had calcium oxalate crystals and 20 samples had numerous epithelial cells. Combi-9 (Medi-Test) used for urinalysis revealed 58 samples (44 females with 14 males) had protein, of which 37 (29 females and 8 males) with growth and 21 without growth. Gram stain besides biochemical tests used for identification of the pathogens, among the positive samples, the most popular pathogens identified as Escherichia coli (49.0%), Klebsiella species (19.6%), Proteus species (11.8%), Pseudomonas aeruginosa (10.8%), and Staphylococcus aureus (8.8%).Regarding to the isolated Gram-negative bacteria, all are resistant to Amikacin (AK), Rifampicin (RA), Nitrofurantoin (F), Trimethoprim + Sulfamethoxazole (SXT), Novobiocin (NV), Nalidixic Acid (NA), Chloramphenicol (C), Tetracycline (TE), Cefepime (FEP), Bacitracin (B), Erythromycin (E) and Streptomycin (S). However, some isolates were less resistant to Amoxicillin (AX), Ceftizoxime (ZOX), Cefoxitin (FOX), Gentamicin (GM), and Cefotaxime (CTX). Gram-positive isolates were resistant to all antimicrobials except Amikacin, Trimethoprim + Sulfamethoxazole, Gentamycin and Chloramphenicol. Females infected with UTIs more than males in Basrah, South Iraq. E. coli was the major isolate, as found in other studies.