ISSN: 0973-7510

E-ISSN: 2581-690X

Ravi Kiran Purama, Mayur Agrawal, Avishek Majumder, Shadab Ahmed and Arun Goyal
Department of Biotechnology, Indian Institute of Technology, Guwahati – 781 039, India.
J Pure Appl Microbiol. 2008;2(1):139-146
© The Author(s). 2008
Received: 18/02/2008 | Accepted: 25/03/2008 | Published: 30/04/2008
Abstract

Four glucansucrase producing Leuconostoc species Leuconostoc mesenteroides B-512F and B-640, Leuconostoc citreum B-742 and Leuconostoc dextranicum B-1146 were characterized for their antibiotic resistance, carbon source utilization, plasmid profiles and glucan synthesizing activities. All the four species were resistant to the antibiotics cotrimazine, norfloxacin and vancomycin. All four species were sensitive to amoxycillin, bacitracin, carbenicillin, cephalothin, cephatoxamine, chloramphenicol, clindamycin, linomycin, oxytetracyclin and tetracyclin. All the four species utilized glucose, fructose, mannose, melibiose, sucrose and trehalose and did not show any activity towards arabinose, lactose and mannitol. The Leuconostoc strain B-640 was unique in fermenting rhamnose.
The Leuconostoc species B-640 and B-1146 possessed plasmids and both showed sucrose hydrolyzing activity by displaying the polysaccharide formation patterns in native polyacrylamide gels. These data will enable in finding similar glucansucrase producing Leuconostoc species which can prove better species used for industrial applications.

Keywords

glucansucrase, glucan, antibiotic, plasmid, Leuconostoc, carbohydrate

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© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.