ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Martina O. Chukwu1 , Akebe Lurther King Abia2, Eunice Ubomba-Jaswa3, Larry C. Obi4 and John Barr Dewar1

1Department of Life and Consumer science, College of Agriculture and Environmental Sciences, University of South Africa, Corner Christain De wet and Pionner Avenue, 1710 Florida Park. Roodepoort, Gauteng, South Africa.
2Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, South Africa.
3Department of Biotechnology, University of Johannesburg, South Africa. 4Sefako Makgatho Health Science University, Ga-Rankuwa, Pretoria, South Africa.
J Pure Appl Microbiol, 2019, 13 (1): 517-530 | Article Number: 5529 | © The Author(s). 2019 

Received: 03/02/2019 | Accepted: 19/03/2019 | Published: 31/03/2019

This study investigated the antibacterial resistance profiles of E. coli pathotypes isolated from children under five years and drinking water samples collected from the North West Province of South Africa and ascertained the clonality of the isolates. Two hundred and forty-one E. coli isolates were recovered from stool samples of diarrhoeic and non-diarrhoeic children under five years old, and drinking water, using the Colilert-18® Quanti-tray/2000 and Eosin methylene blue agar. The presence of enteropathogenic (eaeA), enterohaemorrhagic (eaeA,stx1, stx2 and flicH7), enteroaggregative (eagg), enteroinvasive (ipaH) and enterotoxigenic (ST and LT) E. coli pathotypes were also investigated using PCR. Antibiotic susceptibility was carried out through the disk diffusion method. The presence of blaCTX-M, blaSHV, blaCMY, and blaDHA genes that code for b-lactamases was investigated using real-time PCR. Similarities between human and water isolates were tested using ERIC-PCR. Overall, EHEC (35.8%), EPEC/EHEC (22%), ETEC (21.6%) and EIEC (20.2%) were detected. The highest antibiotic resistance was detected to Clarithromycin (100%) and Erythromycin (100%) while the lowest resistance was against Gentamicin (0.4%). Also, 100% sensitivity was recorded to imipenem and meropenem. Multi-antibiotic resistance was observed in all the pathotypes, and the ESBL genes were detected in 71.6% of the pathotypes. ERIC-PCR indicated 100% similarities in some water and human samples. Pathogenic E. coli is amongst the diarrhoea-causing agents in the North West Province with EHEC being the most identified pathotype. The clonal relatedness of the human and water isolates suggests that domestic water might be a route of transmission.


E. coli, Stool samples, Antibiotic resistance, children.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.