ISSN: 0973-7510

E-ISSN: 2581-690X

Munirat A. Idris , Mohammed S. Jami, Mohd I. Abdul Karim, Parveen Jamal and Suleyman A. Muyibi
Bioenvironmental Engineering Research Centre (BERC), Biotechnology Engineering
Department, Faculty of Engineering, International Islamic University Malaysia (IIUM),
P.O. Box 10 Jalan Gombak, Kuala Lumpur, 50728, Malaysia.
J Pure Appl Microbiol. 2014;8(Spl. Edn. 1):845-851
© The Author(s). 2014
Received: 08/01/2014 | Accepted: 24/03/2014 | Published: 31/05/2014
Abstract

In this research, the antibacterial property of both crude and membrane processed seed extracts were investigated against Escherichia coli, Pseudosomonas aeruginosa Bacillus subtilis and Staphylococcus aureus. The defatted seeds were prepared with water and the water extract obtained was passed through 0.45µm microfiltration membrane system. The antibacterial activity was done using the agar well diffusion. The effect of concentration of the extracts were determined, the Minimum Inhibitory Concentration (MIC) as well as Minimum Bactericidal Concentration (MBC) was carried out. The result showed that the diameter inhibition zone ranges from 13mm to 30mm and increase in the seed extracts showed a corresponding increase in the inhibition of the bacterial strains. Statistical difference was found (P = 0.002) when the mean of antibacterial activity of the membrane processed seed extract against the bacterial strains was compared with the crude seed extract. Statistical results showed that membrane processed extract was more effective than the crude extract.  The results of MIC and MBC values of membrane processed extract seed obtained shows that it was bactericidal against Pseudosomonas aeruginosa, Bacillus subtilis, and bacteriostatic against Escherichia coli Staphylococcus aureus respectively.

Keywords

Moringa oleifera, microfiltration, antibacterial activity,  MIC, MBC

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.