ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Syarifa Fitria1, Zolkafle Buntat2, Zainuddin Nawawi3 , Muhammad Abu Bakar Sidik3, M.I. Jambak3 and Dwirina Yuniarti3

1Graduate School of Environmental Science, University of Sriwijaya, Palembang, South Sumatra 30128, Indonesia.
2Institute of High Voltage and High Current, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Johor Baru 81310, Malaysia.
3Department of Electrical Engineering, Faculty of Engineering, University of Sriwijaya, Palembang, South Sumatra 30128, Indonesia.
J Pure Appl Microbiol, 2019, 13 (1) : 637-641 | Article Number: 5333 | © The Author(s). 2019 

Received:24/10/2018| Accepted: 07/12/2018 | Published: 31/03/2019

Sterilization is essential for inactivation of microorganisms. There are many methods of sterilization, such as the use of heat or chemical processes. However, some equipment can be damaged by heat and can only be sterilized at low temperatures. Failure to properly disinfect or sterilize equipment may lead to transmission via contaminated objects. This paper presents a sterilization process using ozonized water at a temperature of 29.5°C with gram-negative bacteria (Escherichia coli). The antibacterial effect was examined with various concentrations of ORP (oxidation reduction potential) at 702 mV, 802 mV, 940 mV, 950 mV, and 960 mV. A strong linear correlation was observed between ORP value and the surface area of the antibacterial effect. It was found that increasing the concentration of ORP affects the surface area of Escherichia coli.


Antibacterial, E. coli, ozonated water, oxidation reduction potential.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.