Antibacterial Assay of Withania somnifera

ISSN: 0973-7510

E-ISSN: 2581-690X

Antibacterial Assay of Withania somnifera

Naser M. AbdEIslam¹, Shabir Ahmad² , Riaz Ullah³, Ahteram Bibi², Iftikhar Ahmad², M.S. Mostafa⁴ and Jameel A. Khader¹

¹College of Science Research Center, King Saud University, Riyadh, Saudi Arabia.

²Department of Chemistry Kohat University of Science and Technology Kohat, KPK, Pakistan.

³Department of Chemistry Sarhad University of Science and Information Technology
Peshawar, KPK, Pakistan.

⁴Chemistry Department, Faculty of Science, Jazan University, Saudi Arabia.

J Pure Appl Microbiol. 2013;7(1):791-794

Received: 25/01/2013 | Accepted: 20/03/2013 | Published: 31/03/2013

Abstract

The study was carried out to assess antibacterial bioassay of Withania Somnifera. The antimicrobial activities of the butanol, n-hexane, chloroform, ethyl acetate and aqueous extracts of Withania Somnifera. were investigated. The bacterial strains used were E.coli, C.albican, B. atropeous, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus Aureus and Salmonella. that highest zone of inhibition 14 mm resulted for the ethyl acetate extract of Withania Somnifera against P. aeruginosa and Chlorform extract against E. coli. Other significant results obtained by different crude extracts are 13 mm, 12mm and 11 mm against E. coli, C. albican, B. atropeous, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus Aureus and Salmonella. Aqueous, n-hexane and chloroform extract were found totally inactive against Salamonella

Keywords

Antibacterial activity, Withania Somnifera, Well diffusion Assay

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