ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Priya Mehrishi1 , Priti Agarwal1, Shobha Broor1 and Amisha Sharma2
1Department of Microbiology, SGT Medical College, Hospital and Research Institute, SGT University, Gurugram – 122 505, Haryana, India.
2Department of Microbiology, M.M.Medical College and Hospital, M.M. University Solan – 173229, HP, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 403-413 | Article Number: 6047
https://doi.org/10.22207/JPAM.14.1.42 | © The Author(s). 2020
Received: 18/01/2020 | Accepted: 28/03/2020 | Published: 31/03/2020
Abstract

Antimicrobial resistance to the pathogenic microorganism has been characterized as a public health emergency both in the community and in hospitals. That is why; we need to find alternatives, which could be used as antibacterial agents. Therefore aim of this study is to determine the antibacterial and antibiofilm properties of 4 plant extracts Clove (Syzygium aromaticum), Tea (Camellia sinensis), Garlic (Allium sativum), coriander (Coriandrum sativum).Antibacterial properties of plant extracts at different concentrations (50, 25, 12.5, 6.25 mg/mL) were tested against Multi Drug Resistance biofilm producing Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus epidermidis and Staphylococcus saprophyticus using the agar well diffusion method.Minimum Bactericidal Concentration (MBC) and antibiofilm properties of the plant extracts were determined using the tube dilution method and modified crystal violet assay, respectively. Total of 180 clinical isolates were screened for their MDR Pattern. Out of these, 72 were MDR isolates. These MDR isolates were categorized into weak, moderate and strong biofilm producers. Fourteen, Forty nine and nine were weak, moderate and strong biofilm producers, respectively. Out of the 4 plant extracts, Syzygium aromaticum and Camellia sinensis were found to be more effective with maximum zone of inhibition (20 – 25 mm), MBC 6.25 mg/ml and biofilm reduction of more than 50% compared to Allium sativum and Coriandrum sativum. All medicinal plant extracts were effective at different concentrations against the biofilm producing MDR isolates but Syzygium aromaticum and Camellia sinensis showed maximum antibacterial and antibiofilm activity.

Keywords

Antibacterial and Antibiofilm activity, Multi Drug Resistant Bacteria, Plant extracts.

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