Antibacterial activity of petroleum ether, acetone extracts and steam distillation oil of N. sativa seed were tested against pathogenic bacteria. The oil obtained by solvent extraction such as petroleum ether, acetone extract and steam distillation showed significant antibacterial activity which was more against Gram-positive then Gram-negative bacteria. Among Gram-positive bacteria tested, Staphylococcus aureus were more sensitive to oil then Gram-negative bacteria tested Pseudomonas aeruginosa and Escherichia coli. The oil obtained by petroleum extract has significant antibacterial activity compared to acetone extracts and steam distillation oil of N. sativa and related to Ampicillin, Tetracycline, and Ciprofloxacin.
N. sativa L. Seed oil, Antibacterial, Pathogenic Bacteria
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