ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Emad Mohamed Abdallah1 and Eman Ramadan Elsharkawy2,3
1Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia.
2Department of Eco- physiology, Desert Research Center, 15753, Cairo, Egypt.
3Chemistry Department, Faculty of Science, Northern Borders University, Saudi Arabia.
J Pure Appl Microbiol, 2019, 13 (2): 1063-1068 | Article Number: 5439
Received: 28/01/2019| Accepted: 13/04/2019 | Published: 21/06/2019
Abstract

Fruits of Platycladus orientalis growing wild in Arar region, Saudi Arabia was investigated for its antibacterial activity against Staphylococcus saprophyticus. A bacterium associate with serious urinary tract infections (UTI) and cystitis in young human females. GC-MS analysis discloses the presence of some bioactive compounds, such as cupressene, podocarpa-8,11,13-triene, kauran-16-ol, kerruginol, glycoside compounds, cymarin and Periplocymarin which could be attributed to the antibacterial efficacy of that plant product. The extract showed remarkable antibacterial activity against Staphylococcus saprophyticus, the disc diffusion test recorded 16.0±1.0mm zone of inhibition, with relative percentage inhibition (RPI) of Platycladus orientalis extract was 66.6% compared with the chloramphenicol. The MIC and MBC values were 12.5 mg/ml and 50 mg/ml, respectively. The MBC/MIC ratio was 4, indicating that the extract has a good bacteriostatic effect. Therefore, the fruits of Platycladus orientalis could be used in the formulation of antibacterial drugs against Staphylococcus saprophyticus associated with UTI infections.

Keywords

Platycladus orientalis, GC-MS, antibacterial, Staphylococcus saprophyticus.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.