The aim of the present study was to assess the anti-MRSA and anti-VRE activity of ethyl acetate (EA) extract of Streptomyces sp. VITBRK3 and identification of bioactive compounds. The EA extract obtained from the isolate was screened for anti-MRSA and anti-VRE activity by well diffusion method against six drug resistant strains. The isolate was identified by molecular taxonomic characterization. The EA extract was subjected to HPLC-DAD analysis for the identification of antibiotics. The EA extract of VITBRK3 showed the inhibition zone of 19 mm against Staphylococcus aureus 21mm against Enterococcus faecalis. Similarly 20 and 18 mm zone of inhibition was observed against Entrococcus faecium-BM4107 and Entrococcus faecium-Bm4147/ (Van A) respectively. The EA extract also showed 16 mm zone of inhibition against MRSA strain, Staphylococcus aureus U2A 2150 and 20 mm against the clinical isolate Staphylococcus aureus. HPLC-DAD analysis showed the presence of strepturidine, anthranilic acid, indole-3-acetic acid and germicidin B compounds. The observed activity of Streptomyces sp. VITBRK3 may be due to the presence of strepturidine, anthranilic acid, indole-3-acetic acid and germicidin B compounds in the EA extract. The results of this study suggest that secondary metabolites produced by Streptomyces sp. VITBRK3 could be used against MRSA and VRE strains.
Methicillin resistant Staphylococcus aureus (MRSA), Vancomycin resistant Enterococci (VRE), Streptomyces sp. VITBRK3
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