The anti-candidal activity and phytochemicals of the leaf and bark of Vitex negundo L. (Verbenaceae) was evaluated against four species of Candida viz. C. albicans, C. krusei, C. parapsilosis and C. tropicalis. Both polar and non-polar extracts viz. petroleum ether, ethyl acetate, ethanol, methanol and aqueous were prepared and studied for anti-candidal activity using agar cup and broth dilution methods. Although all five extracts showed substantial inhibitory action against all tested Candida species, yet maximum activity was observed in ethanol extract of bark. Minimum Inhibitory Concentration (MIC) values for most of the extracts ranged from 0.156 to 2.5 mg/ml; while the least Minimum Fungicidal Concentration (MFC) value was observed at 2.5 mg/ml. Phytochemical analysis exhibited the presence of carbohydrates, tannin and phenolic compound, glycoside, saponin, steroid & sterols and flavonoid in different extracts. These results, exhibit the anti-candidal activity of Vitex negundo L. extracts would have potentials as an herbal treatment of candidiasis. However, the active components responsible for antifungal activity need to be evaluated.