ISSN: 0973-7510

E-ISSN: 2581-690X

Alireza Khodavandi1 , Fahimeh Alizadeh1, Farideh Namvar2,3, Rosfarizan Mohamad3,4 and Chong Pei Pei5
1Department of Microbiology, Yasouj Branch, Islamic Azad University, Yasouj, Iran.
2Department of Medicine, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
3Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
4Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
5Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
J Pure Appl Microbiol. 2014;8(Spl. Edn. 2):349-356
© The Author(s). 2014
Received: 06/09/2014 | Accepted: 27/10/2014 | Published: 30/11/2014
Abstract

Persian Shallot (Allium ascalonicum Linn.) has been proven to possess some antimicrobial properties against some of bacteria, viruses and fungi. Indeed the aim of this study was to investigate the antifungal properties of several Persian Shallot extracts against important Candida species. In the first stage the preliminary screening of antifungal activity was determined by disc diffusion test. The effective extracts (hexane, ethyl acetate, methanol, and water) were proceeded to obtain minimum inhibitory concentration (MIC) using broth microdilution test and time-kill study was also performed at time intervals. Results showed that the Shallot-hexane extract was more effective in terms of Candida species growth. Subsequently, the effects of the extracts on C. albicans biofilm were determined using XTT assay. Finally the expression level of a crucial gene involved in biofilm formation in C. albicans was analysed by semi-quantitative RT-PCR. The MIC was ranged from 5-600 µg/mL for different species of Candida. Moreover in time-kill study, no colonies were observed at 1× MIC after 24 h of incubation period. On the other hand, XTT assay showed the significant reduction of biofilm in Candida-treated by Shallotat level p<0.05. Eventually the expression level of HWP1 was down regulated through the semi-quantitative RT-PCR (p< 0.05). The present study indicates that HWP1 could be one of the possible targets of Shallot to inhibit biofilm in C. albicans.

Keywords

Candida species, Allium ascalonicum Linn., HWP1, Biofilm

Article Metrics

Article View: 0

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.