ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Nidhi Tayal, Priyansh Srivastava and Nidhi Srivastava

Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Sector-125, Noida – 201 313, India.
J Pure Appl Microbiol, 2019, 13 (1): 567-571 | Article Number: 5441

https://dx.doi.org/10.22207/JPAM.13.1.64 | © The Author(s). 2019 

Received: 24/12/2018| Accepted: 15/02/2019 | Published: 16/03/2019
Abstract

Cancer is a major cause of death worldwide and angiogenesis is critical in cancer progression. Development of new blood vessels and nutrition of tumor cells are heavily dependent on angiogenesis. Angiogenesis is the formation of new blood vessels from existing vasculature. There are several “on” and “off” switches that regulate the process. Decreasing or inhibiting angiogenesis can be therapeutic in cancer and other diseases. Thus, angiogenesis is an important process that occurs both during health and disease. This study was conducted to investigate the anti-angiogenic activity of Carica papaya leaf. The anti-angiogenic activity of papaya leaf was evaluated using docking behaviour of known bioactive compounds of leaf as ligands with angiogenic receptors VEGFR1 and VEGFR2 along with their putative binding sites using Swiss Dock Web server (In silico) and further based on docking results leaf aqueous extract was used for implantation in chorioallantoic membrane (CAM) egg yolk angiogenesis model (in vivo). Docking studies and binding free energy calculations revealed that among known bioactive compounds of leaf Ascorbic acid, quercetin, riboflavin and lycopene have maximum free energy as compared to other investigated ligands. Similarly, CAM assay also showed the inhibitory effect of the Carica papaya leaf with respect their reduction in length, size and junctions of blood capillaries compared to untreated egg yolk. The results showed that Ascorbic Acid, Quercetin, Riboflavin and, Lycopene (leaf compounds) can attenuate angiogenesis in pathological conditions and can be potent in drug discovery as well as medical science.

Keywords

Carica papaya, Angiogenesis, Medical Science, Cancer, Pathological, Phytochemical.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.