ISSN: 0973-7510

E-ISSN: 2581-690X

Shubha Trivedi, Mukesh Srivastava, Sonika Pandey, Vipul Kumar, Anuradha Singh, M. Shahid and Yatindra Srivastava
Department of Plant Pathology, C.S. Azad University of Agriculture & Technology, Kanpur – 208 002 India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1591-1598
© The Author(s). 2016
Received: 13/01/2016 | Accepted: 19/04/2016 | Published: 30/06/2016
Abstract

Trichoderma spp. are bio control agents extensively used in management of fungal diseases of crop plants exhibiting  antagonism  against  a  wide   range   of   plant   pathogens.   It has been known from many years that Trichoderma spp. produces wide range of antibiotic substances and they paralyze other fungi. They can also compete for key exudates from seeds that stimulates the germination of propagules of plant pathogenic fungi in soil (Howell, 2002) and more generally compete with the soil microorganisms for nutrient and space. Keeping this in view nine   Trichoderma spp. viz; T.asperellum, T. atroviride, T. harzianum, T. koningii, T. longibrachiatum, T. minutisporum, T. ressei, T. viride, and T. virens isolated from different locations of India were characterized for their antagonistic activity against wilt and dry root rot pathogens of chickpea. The various spp. showed differential reaction against the test pathogens Fusarium oxysporum f.sp. ciceri (Foc) and Rhizoctonia bataticola (Rb). However, T. harzianum was most effective causing 55.0 and 31.8 percent inhibition of mycelial growth in Foc and Rb respectively. Scanning Electron microscopic investigations about hyphal interactions between antagonists and test fungi revealed that the mycoparasitic hyphae were usually attached longitudinally to the hyphae of the pathogen. Coiling of hyphae, short contact branches, hyphal depression and pincer shaped structures were seen during observations through SEM indicated the mode of action in biological control of the test pathogens.

Keywords

Fusarium oxysporum f.sp. ciceri. Rhizoctonia bataticola. Trichoderma spp., Antagonism, Hyphal relationship, Wilt Complex, Chickpea, Biological management.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.