ISSN: 0973-7510

E-ISSN: 2581-690X

Mobolaji Felicia Adegboye1, Olubukola Oluranti Babalola1 , Lubanza Ngoma1 and Anthony Ifenyi Okoh2
1Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, South Africa.
2Applied and Environmental Microbiology Research Group, University of Fort Hare, Private Bag X1314, Alice 57000, South Africa.
J Pure Appl Microbiol. 2012;6(3):1001-1010
© The Author(s). 2012
Received: 02/05/2012 | Accepted: 10/06/2012 | Published: 30/09/2012
Abstract

Streptomyces species were isolated from rhizospheric soils collected from different localities in Mahikeng, North West Province of South Africa. A total of 84 isolates were obtained from 5 rhizospheric soil samples, highest number of isolated were recovered from maize (Zea mays) rhizosphere. The genus Streptomyces was identified by cultural, morphological, physiological and molecular analyses. 26 culturally distinct isolates were identified to the species level by 16S rDNA gene sequence analysis. The sequences of 16S rDNA gene of the isolates matched with Streptomyces but different species in BLAST analysis, similarities ranging between 100%-91%. Rhizosphere can be considered as inexhaustible resource of microorganisms that can be harness for possible exploration.

Keywords

Streptomyces, rhizosphere, characterization, 16S rDNA gene

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.