ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Arijit De1, Souryadeep Mukherjee2, Gobinda Chandra Sadhukhan3 and Nimai Chandra Saha4
1Department of Zoology, Vidyasagar College, Block CL, Plot 3-8 & 44-50, Sector II, Salt Lake, Kolkata, West Bengal- 700 091, India.
2Department of Life Sciences, Presidency University, 86/1, College Street, Kolkata, West Bengal – 700073, India.
3UGC – HRDC, Jadavpur University,  Block – LB, Plot No 8, Sector III, Salt Lake City, Kolkata, West Bengal 700098 Kolkata – 700098, India.
4University of Burdwan, Rajbati, Burdwan, West Bengal – 713104 , India.
J Pure Appl Microbiol. 2018;12(3):1597-1605 | © The Author(s). 2018
Received: 10/06/2018 | Accepted: 26/08/2018 | Published: 30/09/2018

The present study investigates the cellulolytic potential of three prokaryotic strains belonging to genus Bacillus isolated from the benthic soil of two aquaculture farms in East Kolkata Wetlands. For the purpose of identification and phylogenetic analyses, biochemical characterization of these three strains were performed followed by molecular characterization of 16S rRNA sequences. Maximum cellulolytic potential was found in Bacillus cereus Strain SWA6a for both Exo – b,1-4- glucanase and Endo – b,1-4- glucanase with an activity of 0.08214 ± 0.00412 and 0.11263 ± 0.00478 IU ml-1min-1 respectively. The enzyme activity ranged from 0.06112 to 0.08214 IU ml-1min-1 for Exo – b,1-4- glucanase and 0.10018 to 0.11263 IU ml-1min-1 for Endo – b,1-4- glucanase.  Degradation of free insoluble cellulose ranged from 55 to 63% within a time span of 96 hours in pH 6.8 – 7.2 when incubated at 37°C. Overall, the cellulolytic activity of the isolated strains may have strong implications in the field of biological conversion of cellulose-containing municipal wastes, agricultural and forestry wastes. These isolates maintain the equilibrium of the aquaculture pond by maintaining the biogeochemical cycle of carbon and hence has the prospect to be used for bioaugmentation purposes as a step towards a microbial-based aquaculture method.


Aquaculture benthos, Bacillus, Cellulolysis, 16S rDNA Analysis, Cellulase

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© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.