ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
G. Sanjotha1, C.T. Shivasharana1 and Sudheer I. Manawadi2
1PG Studies in Biotechnology and Microbiology, Karnataka University, Dharwad, India.
2Department of Biotechnology, Government Arts and Science College, Karwar, India.
J. Pure Appl. Microbiol., 2019, 13 (4): 2261-2272 | Article Number: 5872 | © The Author(s). 2019
Received: 23/09/2019 | Accepted: 30/11/2019 | Published: 18/12/2019

Biosynthesis of kojic acid by fungi has been acquired huge attention nowadays because it can be used as an alternative method to chemically synthesized compounds. The fundamental feature of the microbiological agitation practice was to obtain potential fungi to achieve greater amount of kojic acid, the fungal isolate used in this study exhibited maximum growth on PDA and was identified as A.flavus based on its microscopic and macroscopic characteristics and ITS sequence analysis. In this vignette, appropriate cultivation conditions were carried out to gain a highest quantity of kojic acid. The maximum yield of kojic acid (31g/l) was reached by formulating the medium. The massive amount of kojic acid and maximum biomass was achieved from A. flavus by utilizing yeast extract as a nitrogen source and carbon source as glucose. The optimum incubation temperature employed was at 30oC, optimal pH was 3. Antagonistic activity was analyzed against both bacterial and fungal pathogens. The ethyl acetate extract exhibit increased antibacterial activity and maximum antifungal activity comparable with methanol, and chloroform.


Aspergillus flavus, kojic acid, antimicrobial activity, optimization, dry biomass.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.