An endophytic bacterial strain from fresh flower of Gardenia jasminoides, ZZB08, was identified as Bacillus vallismortis based on morphologic, physiological, biochemical characteristics, and 16S rDNA sequence analysis. By method of SPME-GC/MS, 17 and 18 compounds with relative content (RT) more than 0.5% were respectively detected from flower and the culture of ZZB08, which covered a wide range of chemical types. Comparatively, five major volatile compounds of flower, linalool, a-farnesene, trans-b-ocimene, z-3-hexenyl tiglate and methyl tiglate, were also found in culture of the ZZB08 but with lower concentrations than that in the flower. This is the first report on the endophyte which could produce fragrant volatiles similar as its host.