ISSN: 0973-7510

E-ISSN: 2581-690X

Behrooz Yahyaei1, Sepideh Arabzadeh2 and Parastoo Pourali1

1Department of Medical Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
2Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
J. Pure Appl. Microbiol. 2014, 8(6):4495-4501
© The Author(s). 2014
Received: 08/08/2014 | Accepted: 30/10/2014 | Published: 31/12/2014

Major purpose of this study was to explore an alternative and simple method for silver and gold nanoparticles bio-production by Escherichia coli. In the previous (routine) technique for nanoparticles bio-production, after culturing the bacteria, the bacterial culture supernatant was treated with aqueous solutions of silver nitrate and chloroauric acid ions. To perform bio-synthesis of silver and gold nanoparticles by the alternative method, unlike the routine one, the bacteria were subjected to the toxic metal ions at their MICs (minimal inhibitory concentration) immediate after inoculation to the medium. Bio-production of nanoparticles by these two methods was monitored through visible spectrophotometry, transmission electron microscopy (TEM) and X-ray diffraction analysis (XRD). The results showed that silver and gold nanoparticles were produced by both types of the routine and alternative techniques but the rate of bio-production was relatively more by the alternative one. Moreover, this type of bio-production is simpler, faster and cleaner than the previous one.


Bio-production, Silver nanoparticles, Gold nanoparticles, Alternative method

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.