ISSN: 0973-7510

E-ISSN: 2581-690X

Ronak Prajapati1 , R.V. Vyas1, H.K. Patel2 and H.N. Shelat1
1Department of Agricultural Microbiology and Biofertilizers Project, Anand Agricultural University, Anand – 388 110, Gujarat, India.
2AICRP-Weed Management, B.A.College of Agriculture, Anand Agricultural University,
Anand – 388 110, Gujarat, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2289-2298
© The Author(s). 2016
Received: 10/04/2016 | Accepted: 24/06/2016 | Published: 30/09/2016
Abstract

A 16S rRNA gene-based fingerprinting method was developed for the preliminary characterization of native diazotrophs bacterial isolates Azotobacter, Acetobacter and Azospirillum tested with reference strains belonging to the free-living nitrogen-fixing bacterial group. According to this method, the 16S rRNA gene was amplified using universal primers and PCR products were subsequently digested with TaqI, BamHI, HinfI and HaeIII. The analysis of the restriction profiles obtained showed that the method is able to define a unique species-specific phylotype (SSP) for each of the native diazotrophic bacterial isolates. Phylogenetic analysis of 16S rRNA indicated that these diazotrophic bacterial isolates belonged to respective genus Azotobacter, Acetobacter and Azospirillum and closely related to standard strains.

Keywords

Diazotrphs, 16s rRNA, Restriction enzyme, Azotobacter, Acetobacter, Azospirillum.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.