Aminoglycoside Resistance in Clinical Klebsiella pneumoniae Isolates

ISSN: 0973-7510

E-ISSN: 2581-690X

Aminoglycoside Resistance in Clinical Klebsiella pneumoniae Isolates

Xiaolong Tang^1,2, Shuyu Cai^1,2, Jia Chen⁴, Lifa XU¹, Jian Wang¹ , Wenyue Li¹, Xiuyun Zhang¹, Lin Sun¹, Liyi Fang¹, Xin Zhou¹, Zhenyou Jiang³, Peng Liu¹ and Liuhua Wei⁵

¹Stem cell Engineering Research Center, School of Medical, Anhui University of Science & Technology, Huainan – 232 001, China.

²Department of Laboratory Medicine, Tianjin Medical College, Tianjin – 300 222, China.

³Department of Traditional Chinese Medicine, School of Medical, Jinan University, Guangzhou – 510 632, China.

⁴Department of Microbiology and Immunology, School of Medical, Jinan University, Guangzhou – 510 632, China.

⁵Clinical Laboratory, Liuzhou workers Hospital, Guangxi – 545 005, China.

J Pure Appl Microbiol. 2013;7(4):2993-3000

Received: 23/09/2013 | Accepted: 06/11/2013 | Published: 30/12/2013

Abstract

To analyze the Klebsiella pneumoniae (K. pneumoniae) susceptibility to aminoglycosides antibiotics in clinical isolates in Guangzhou, and to characterize the molecular resistance mechanisms of K. pneumoniae against aminoglycosides (AGs) agents. We examined 1495 strains clinical isolates of Klebsiella pneumoniae from Microbiology Laboratory of the Guangdong Provincial Traditional Chinese Medicine in China between 2008 and 2012 by MicroScan Wa1kAway-96. And detected Minimal inhibitory concentrations (MICs) of amikacin, gentamicin and tobramycin to K. pneumoniae by agar dilution methods. Isolates were analyzed by polymerase chain reaction (PCR) amplification techniques to determine whether six aminoglycoside modifying enzymes (AMEs) genes ( aac(3)-a!, aac(6′)-Ib, ant(3″)-I, ant(2″)-I, aac(3)-I, aac(6′)-a!)and six 16S rRNA mehtylases genes (armA, rmtA, rmtB, rmtC, rmtD, npmA) were present. Additionally, 16S rRNA methylase gene positive isolates were chosen for a plasmid transfer test to explore whether the resistance marker was located on the transfer plasmid. Pulsed-field gel electrophoresis was used for genotyping 16S rRNA methylase gene positive isolates. A total of 265 isolates was identified as resistant to one or more aminoglycosides, with resistance rates to amikacin, tobramycin and gentamicin of 4.15%(62/1495), 9.50%(142/1495), and 16.05%(240/1495), respectively. Among the 265 resistant aminoglycoside isolates, 84.5% carried resistance genes (180 isolates carried AMEs and 44 isolates carried both AMEs and methylase genes). Aminoglycoside resistance in Guangzhou clinical isolates of K. pneumoniae is still low, and kept stable over the last 5 years; frequency of AMEs and 16S rRNA methylase in K. pneumoniae was low.

Keywords

Klebsiella pneumoniae, aminoglycosides, aminoglycoside modifying enzymes (AMES), 16S rRNA methylase, antimicrobial resistance, gene

Download PDF

Article Metrics

Article View: 901

JPAM.7.4.64 (241 downloads)

Share This Article

© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.