ISSN: 0973-7510
E-ISSN: 2581-690X
Low-toxicity transgenic castor (Ricinus communis) plants were produced through Agrobacterium-mediated genetic transformation. Ricin, which is highly toxic, affects the application of castor. In this research, a 351-bp fragment located at ricin A chain gene (RTA) was selected to establish an RNA interference construct that was introduced into epicotyl of castor, thus transformants were obtained. Through semi-quantitative analysis on RTA, it was confirmed that the RTA expression quantity of the transformants was apparently decreased, demonstrating the interference efficiency of the transformation vectors and their function of reducing the expression of ricin at the RNA level. An improved protocol for the transformation and regeneration of less toxic caster has been developed for the first time.
Castor, ricin, Agrobacterium tumefaciens, RNAi
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