Chicken drumstick mushroom (Coprinus comatus (O.F. Mull) Pers.) is a medicinal mushroom containing various mycochemical compounds, such as flavonoids, terpenoids, vitamins C and E, quercetin, and rutin. These bioactive compounds have pharmacological activities, including anti-nephrotoxic, decreasing uric acid (UA) level, anti-inflammatory, and antioxidant, capable of reducing free radicals. Therefore, this study aims to investigate anti-inflammatory and antioxidant activity of C. comatus ethanol extract on carrageenan-induced inflammatory rats. A completely randomized design (CRD) was used, and the test rats were divided into 6 groups. These included 1 healthy group (HG), 5 groups induced by 0.5 mL of 1% carrageenan, in which 3 were administered extract at doses of 250 (TA1), 500 (TA2), and 750 mg/kg BW (TA3), 1 given sodium diclofenac (PG), and 1 served as a negative control (NG). During the procedure, extract administration was carried out for 14 days. The results showed that 500 mg/kg BW dose reduced IL-1β (12.38%), MDA (20.72%), and UA (16.67%), as well as increased superoxide dismutase (SOD) (12.79%). Meanwhile, 250 mg dose increased total protein (TP) (5.33 g/dL) and globulin (GLB) (1.58 g/dL), with 750 mg dose also elevating albumin (ALB) (3.89 g/dL). Increased enzymatic antioxidants can prevent oxidative stress and peroxidation reactions. Antioxidant effects of C. comatus are expected to decrease the release of inflammatory mediators, leading to the prevention of cell and tissue damage caused by oxidative stress.