Often referred as the “jewel of the basin” the ocean contains a vast array of pivotal bioactive compounds from both living and non-living sources. The macroalgae have been regarded as a pool diversity of marine environment. They provide number of natural bioactive compounds to alleviate human ailments and thousands of them are isolated from distinct species and various places. Recently pharmacological industries paid attention to discovering various low side effect bioactive compounds from marine sources, especially macroalgae. The present study collected few red algae like Gelidiella acerosa (Forsskal), Acanthophora spicifera (Vahl) and Hypnea musciformis (Wulfen) from the low tide region in Pudumadam coast, Gulf of Mannar; Centroceras clavulatum (C. Agardh) and Gracillaria corticata (J. Agardh) from Pamban coast, Gulf of Mannar (GoM) and Galaxaura obtongata from Villunditirtham coast, Palk Bay and Laurencia papillosa (C. Agardh) (Greville) from the Olaikuda coastal region, Rameswaram, Palk Bay, Tamil Nadu, India and subjected for bioactive principles screening using various solvents as these compounds would be of a source for new drug candidates. Further, these seven methanol crude extracts examined for their anti-microbial potential using human pathogens. Our findings revealed stronger anti-microbial potential of methanol extracts of Gelidiella acerosa, Acanthophora spicifera and Hypnea musciformis. The results of the present study confirmed the potential use of marine algae as a reliable source of antibacterial agents. Based on these results, further purification, and identification of individual compounds required as they are specific for anti-microbial activity may pave way for the development of novel therapeutics to treat illness caused by microbes. Besides, further investigations are crucial to elucidate the structure and specific molecular mechanisms of these bioactive components which are responsible for various additional pharmacological activities.