Paracetamol is a widely recognized pharmaceutical contaminant in the environment, emphasizing the need for effective biotransformation strategies for its remediation. This study explored the biotransformation ability of Alcaligenes sp., isolated from waste-burned soil, using high performance liquid chromatography analysis. Bacterial isolation was performed on nutrient agar containing 50 mg/L paracetamol as the sole carbon source, followed by identification through Gram staining and ALSP testing. Paracetamol biotransformation was conducted in liquid nutrient medium and incubated at 30 °C on a shaker for 72 hours. Three bacterial isolates (RZP1, RZP2, and RZP3) were obtained, with RZP2 and RZP3 confirmed as Alcaligenes sp. All isolates were Gram-negative and exhibited distinct morphological characteristics. Thin layer chromatography showed varying Rf values for each isolate: RZP1 (0.48), RZP2 (0.58), and RZP3 (0.74), compared to 0.70 for the paracetamol standard. HPLC analysis revealed a complex biotransformation profile, with five metabolite peaks detected at 1.657, 2.418, 2.975, 5.311, and 8.225 minutes, whereas the paracetamol standard exhibited a single peak at 5.71 minutes. The total metabolite peak area reached 76,184,224, indicating extensive biotransformation involving both hydrolytic and oxidative pathways. These results demonstrate that Alcaligenes sp. can biotransform paracetamol into multiple metabolites with diverse polarity profiles, highlighting its potential application in environmentally sustainable pharmaceutical bioremediation.