ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Neeta Bhagat1, Monika Yadav1, Roopal Dhar1, Richa Kothari2, Swati Allen3, Sohini Singh1 and Tanu Allen1
1Amity Institute of Biotechnology, Amity University Uttar Pradesh-Noida, India.
2Department of Environmental Sciences, Central University of Jammu, Rahya Suchani (Bagla), Samba, Jammu and Kashmir, India.
3Indian Council of Medical Research, New Delhi, India.
Article Number: 10834 | © The Author(s). 2026
J Pure Appl Microbiol. 2026;20(1):414-428. https://doi.org/10.22207/JPAM.20.1.27
Received: 03 August 2025 | Accepted: 27 November 2025 | Published online: 25 February 2026
Issue online: March 2026
Abstract

The growing concern over environmental pollution caused by petroleum and oil spills, as well as the accumulation of hydrocarbons in soil and aquatic bodies, has intensified sustainable bioremediation strategies. Petroleum hydrocarbons pose a serious ecological and public health hazard due to their toxic and bioaccumulative nature. In contrast to conventional physical and chemical methods, bioremediation with hydrocarbon-degrading bacteria is eco-friendly and cost-effective in addressing environmental pollution. In the current research, we isolated and characterized four bacterial species from soil samples collected from salt lakes in Gujarat, evaluating their ability to degrade different hydrocarbons under varying salt concentrations. All four isolates (T1, T2, T3, T4) were found to grow in the presence of petrol-supplemented medium, and two of the isolates (T2 and T3) grew well in the presence of other hydrocarbons such as benzene, toluene, naphthalene, phenol, and diesel. They could tolerate up to 15% salt in the media and could degrade petrol and diesel into salt-supplemented media. All four isolates demonstrated biofilm-forming ability in the presence of various hydrocarbons in the media. The petrol degradation efficiency of the isolates, both in the presence and absence of salt in the media, demonstrated high petrol-degrading efficiencies. These isolates showed motility and endospore formation and were gram-positive rods belonging to the Bacillus and Paenibacillus genera.

Keywords

Bioremediation, Hydrocarbon-degrading Bacteria, Petroleum, Biofilm

Article Metrics

Article View: 212

Share This Article

© The Author(s) 2026. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.