The amount of petroleum hydrocarbons affects biodegradation. Different classifications can be used to petroleum components Asphaltenes, ethers, fatty acids, porphyrins and resins saturated fatty acids. Twenty soil samples tainted with petroleum products and crude oil were gathered from the Basra oil fields and other parts of the Babylon governorate; of these twenty samples, seventeen showed Pseudomonas aeruginosa (P. aeruginosa) growth on the chromogenic agars, which manifested as purple colonies. Using 16S rRNA and PCR technology with a 505 bp PCR product size, P. aeruginosa isolated from soil contaminated with petroleum products was identified. The Kirby-Bauer disk diffusion susceptibility test, a method for testing antibiotic sensitivity, produced the following findings. The percentages of ciprofloxacin CIP, imipenem IPM, meropenem MEM, tobramycin TOB and amoxicillin + clavulanic acid AMC equal to 10 mg, ceftazidime CAZ (30 mg), and cefotaxime CTX (30 mg) are 59%, 70%, and 82% respectively. The goal of creating a minimum salt media and introducing diesel at 0.05% concentration is to ascertain how well bacteria may grow in petroleum product-containing settings by breaking down diesel. For a total of 21 days, the optical density in this test is measured every seven days. The results show a rise in the optical density, which suggests that the culture medium being used is growing more quickly. Additionally, it was noted that the center’s color and turbidity was altered. P. aeruginosa can grow in soil contaminated with petroleum products, had the ability to degrade petroleum pollutant, highly resisted to antibiotic that used in this study.